What A busy Lab day!!!!!!!

Wha! I've never been so busy before...

4 hours straight in the lab--> doing 3 experiments in one go.. Of course you need to plan how to stagger your experiments. So I started out very early... I thought I was the earliest..... but guess what, See Jye was there too at 810am! wha.. He want to fight with me for the queue of the CEDEX machine.

So tiring man.. I almost did a wrong thing.. which is to put my "promoted" batch of sample into the same incubator. Then I quickly shifted them to the shaking incubator... "phew.."

After that. I went to collate my results from the CEDEX machine. WHa!!! What the.... The number of dead cell was tremendous.

Second Harvest
Batch 1
Date: 20/3/08
**with 1.5X dilution

I
ID
IDP
IDA
IDPA
Viable
7.80E+04
2.87E+05
2.56E+05
4.30E+04
5.80E+04
Total
3.66E+05
7.17E+05
4.25E+05
3.99E+05
3.77E+05
Viability
21.40%
40.10%
60.20%
10.70%
15.30%
Agg
7.9
10.2
28.2
2.8
7.7
Std Dev
1.34
2.25
2.05
1.02
1.2
Actual Viability
1.17E+05
4.31E+05
3.84E+05
6.45E+04
8.70E+04
Remarks
Cycle shorten
6 Well
6 Well
same
same

At first look.. I got a shock. But then I realised that it is only natural.. Furthermore I forgot to time 1.5 due to the dilution. How come so blur???
Basically, I've got my batch split up into 3, which basically more work and more overtime.. Have to come back on saturdays and sundays..

Oh Dr Ng is back but he gotta rush off for some meeting. Haiz. Later I will report to him... Too busy man! OK I better go and prepare my enzymes for ELISA>>>> no time to waste!
Bye